All BGRI Abstracts

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Wheat disease surveillance and monitoring in Bangladesh

BGRI 2018 Poster Abstract
Md Farhad Wheat Research Centre, Bangladesh Agricultural Research Institute
Kishwar-E-,Mustarin, Md Mostofa Ali, Reza, Krishna Kanto, Roy, Md. Ashraful, Alam, Md. Rezaul, Kabir, MD Abdil, Hakim, Md Monwar, Hossain, Md Rabiul, Islam, Tim, Krupnik, Md Forhad, Amin, Md. Mosharraf, Hossain, Nure Alam, Siddque, Paritosh Kumar, Malaker, Maricellis, Acevedo, Noresh Chandra Deb, Barma

Disease surveillance and monitoring has been regularly organized by Wheat Research Centre (WRC), in major wheat growing areas of Bangladesh since 2010-11 to track the current status of common diseases, first outbreak of new disease(s), the pathogen hotspot and identify new virulent races. Besides the paper based traditional survey, scientists of WRC are now using different tools like Smartphone/Tablet with supporting applications. Several trainings were arranged under DGGW project on rust tool box in Bangladesh. Among all smartphone applications, RustSurvey is the easiest and handy application which integrates with the SAARC Surveillance Toolbox.
A disease surveillance program on wheat blast was organized in Mid February 2017 followed by hands on training in collaboration with CIMMYT and CU, USA. Out of 103 sites surveyed, 33 sites were found infected with wheat blast. Overall disease incidence was comparatively lower than the previous season with low disease severity (5-10%). Surveillance program on rust diseases was also conducted in early March 2016. Among 102 rust survey sites stem rust and yellow rust was not found, but leaf rust occurred with varying levels of severity depending on field locations, sowing times and cultivars grown. About 52% of the 102 fields investigated had leaf rust, and almost 73% of the infected fields showed low (<20%), 21% moderate (20-40%) and only 6% showed high (more than 40%) disease severity. Timely (15-30 November) planted crops largely escaped or had less disease compared to those planted late in the season. The predominant cultivar Prodip as well as BARI Gom 25 and 26 showed zero to high disease levels with MSS type reactions. BARI Gom 21, 28, 29 and 30 were free from leaf rust infection. Furthermore, Spot blotch was found in most of the region with low to high level field incidence depending on crop growth stage.


Achieving triple rust resistance in wheat through combination of phenomic and genomic tools

BGRI 2018 Poster Abstract
Urmil Bansal University of Sydney Plant Breeding Institute
Naeela,Qureshi, Vallence, Nsaiyera, Pakeer, Kandiah, Mesfin, Gesesse, Mandeep, Randhawa, Mumta, Chhetri, Bosco, Chenayek, James, Kolmer, Miroslav, Valarik, Zaroslav, Dolezel, Beat, Keller, Matthew, Hayden, Justin, Faris, Harbans, Bariana, Vanessa, Wells

Dr. Norman Borlaug stated that rust never sleeps and this enables rust pathogens to produce new strains capable of putting rust resistance genes to rest. These pathogens continue to pose threats to global wheat production. Wheat breeders have made significant progress to control rust outbreaks using conventional selection technologies; however, some critical shifts in pathogen populations have let them down. Rapid evolution in molecular marker technologies in the last 15 years and refinement of phenomic facilities have expedited the process of discovery and characterisation of rust resistance genes to underpin the development and validation of markers closely linked with genetically diverse sources of resistance. A high proportion of the formally named rust resistance genes were characterized in the 21st century and markers closely linked with these genes have been developed and validated. The marker tagged sources of resistance to three rust diseases have equipped the wheat breeding community with tools to deploy combinations of all stage and adult plant resistance genes in future wheat cultivars. The question that whether we have enough resistance genes discovered to compete against the ever-awake rust pathogens. In our opinion, we cannot be complacent and discovery needs to continue to ensure food security. This presentation will discuss the role of advances in phenomic and genomic technologies to achieve durable rust control in wheat.


Durum wheat adaptation and yield formation as affected by Ppd-1 photoperiod sensitivity genes

BGRI 2018 Poster Abstract
Dolors Villegas Institute of Agriculture and Food Research and Technology
Karim,Ammar, Susanne, Dreisigacker, Jos? Mar?a, Arjona, Conxita, Royo, , , , , , , , , , , , , , , , , , , , , ,

Understanding the effect of genetic factors controlling flowering time is crucial to fine-tune crop adaptation to each target environment and maximize yield.
A set of spring durum wheat inbred lines carrying all but one of the possible allelic combinations at Ppd-A1 and Ppd-B1 genes was developed through a collaboration between IRTA and CIMMYT. The collection was grown during several years at four sites at latitudes ranging from 19?N to 41?N in order to assess the effect of Ppd-1 genes on development, biomass production and allocation, as well as grain yield formation.
Environmental constraints were responsible for most of the observed variation for flowering time and yield components. Latitude was a main driver of flowering time, which was later in northern sites and associated with lower minimum temperatures before flowering. Data on environmental constraints explaining a large proportion of grains m-2 and kernel weight variation will be presented. The effect on flowering time of Ppd-A1 alleles conferring photoperiod insensitivity was enhanced at sites with average daylength before flowering lower than 12h. Ppd-A1 caused a stronger effect on flowering time than Ppd-B1, which was found responsible for differences in grains m-2, associated with longer photoperiods from double-ridge to terminal spikelet stages. These differences in grains m-2, however, did not result in higher yields due to kernel weight compensation. Late flowering genotypes carrying alleles conferring photoperiod sensitivity had greater biomass at anthesis but it did not confer superior yields. Early flowering times were associated with higher yields in autumn-sowing sites due to a large contribution to yield of current photosynthesis during grain filling. Early flowering genotypes tended to yield more due to higher kernel weights, and the interaction of allele combination x environment will be discussed in the context of using allelic information as environment-specific guideline in breeding efforts.


Mapping resistance in wheat landraces to the Ug99 race group of the stem rust pathogen

BGRI 2018 Poster Abstract
Kerri Neugebauer USDA-ARS
Ebrahiem,Babiker, Tyler, Gordon, Sam, Stoxen, Matthew, Rouse, Yue, Jin, Shiaoman, Chao, John, Bonman, , , , , , , , , , , , , , , ,

Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a threat to wheat production worldwide. To manage this important disease, new sources of genetic resistance are needed and common wheat landraces are a potential source of such resistance. Landrace accessions from the USDA-ARS National Small Grains Collection were evaluated for seedling resistance to the Ug99 race group. To identify accessions most likely to carry novel resistance genes, a bulked segregant analysis (BSA) approach was used. Seven resistant accessions were crossed to a susceptible parent line and F3 families were tested against Pgt race TTKSK. The resistant plants were identified and grouped into two bulks per population. The bulks, along with the parents and F1 progeny, were genotyped with the 90K wheat iSelect SNP genotyping platform. Four of the populations appeared to segregate in a 1:1 phenotypic resistant/susceptible ratio, one in a 1:2 ratio, and two in 1:3 ratios. However, chi squared tests indicated the ratios were statistically the best fit for only two of the 1:1 segregating populations and one of the 1:3 segregating populations. Initial BSA results indicate the markers associated with reduced stem rust infection are located on wheat chromosomes 1DL and 2B. These mapping populations are being advanced for further evaluation to ascertain if novel resistance to the Ug99 stem rust race group is present.


Aecial infection status of Berberis spp. in Kastamonu province of Turkey

BGRI 2018 Poster Abstract
Nil?fer Akci Central Research Institute for Field Crops, Yenimahalle, Ankara, Turkey
Aziz,Karakaya, , , , , , , , , , , , , , , , , , , , , , , , , , , ,

Berberis species are important alternate hosts and generate new races of stem rust fungus, Puccinia graminis f. sp. tritici and yellow (stripe) rust fungus Puccinia striiformis. Berberis species are common in Kastamonu province of Turkey. In 2016 and 2017, surveys were conducted in Kastamonu province in order to elucidate aecial infection status of Berberis species in this region. In 2016, the central region and A?l?, Ara?, Daday, ?hsangazi, Seydiler, Ta?k?pr? and Tosya regions and in 2017 central region and Ara?, Daday, Han?n?, P?narba??, Seydiler, Ta?k?pr? and Tosya regions of Kastamonu province were investigated. It appears that there are at least two different Berberis species exist in that area. Berberis species showed variation in terms of fruit color and morphological characters. In 2016, 50 Berberis plants were examined and aecia were present in 38 plants (76%). Percentage of plants parts infected with aecia ranged between 3-80%. In 2017, 64 Berberis plants were examined. Aecia were present in 34 plants (53%). Percentage of plants parts infected with aecia ranged between 3-85%. Aecia were mainly observed on leaves but also observed on other plant parts including flower parts, fruit and young twigs. The role of these aecia and Berberis spp. on rust diseases in Kastamonu province of Turkey should be investigated.

This study was supported by General Directorate of Agricultural Research and Policies, Turkey (Project No: TAGEM-BS-15\12-01\02-02).


Assessment of slow rusting of landraces of bread wheat to <em>Puccinia striiformis</em> f.sp. <em>tritici</em> under artificial field inoculation

BGRI 2018 Poster Abstract
Fedaa Alo ICARDA
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Yellow rust caused by Puccinia striiformis f.sp. tritici, is the most devastating fungal disease of wheat, especially in CWANA region. Growing cultivars with durable resistance is the most economical control measure. A field study was conducted to evaluate 500 bread wheat landraces along with the susceptible control ?Morocco? using artificial inoculation under field conditions at Tel Hadia, Syria during 2010-11 and 2011-12 growing seasons. The most prevailing yellow rust virulent race 70E214 was used for artificial inoculation. The disease scoring started when the disease severity was more than 50 % on the leaves of the susceptible check ?Morocco? and continued for four scorings at the intervals of 7 days. Slow rusting resistance was assessed based on the development of disease over time using the Area under Disease Progress Curve (AUDPC), Coefficient of Infection (CI), Final rust Severity (FRS), Infection Rate ?(r)? and Relative Resistance Index (RRI). None of the landraces showed immune reaction and 10% showed lowest values for all parameters, suggesting that resistance in these landraces was controlled by major genes. Approximately 65% of landraces were marked as having different levels of slow rusting and 25% were highly susceptible. Cluster analysis based on partial resistance parameters revealed two major clusters: Susceptible and low level of slow rusting were grouped in the first cluster; Resistant, high level and moderate level of partial resistance were grouped in the second cluster.
By comparing the results obtained from RRI and others parameters, we found that landraces with very low values for all parameters exhibited high RRI value of 9, while those that showed high, moderate and low levels of slow rusting, had RRI ranges of 8-9, 7-8 and 5-7, respectively. The landraces with maximum values from each parameter showed very low RRI values of less than 5.


Business mentoring contributes to the growth of small and wheat seed enterprises in Nepal

BGRI 2018 Poster Abstract
Narayan Khanal CIMMYT International, South Asia, Regional Office, Kathmandu
Gurbinder,Gill, Madan, Bhatta, Andrew, McDonald, Arun, Joshi, , , , , , , , , , , , , , , , , , , , , ,

The experience of seed system development works in Nepal shows that lack of business orientation is one of the key challenges for the growth of Nepalese seed enterprises (SEs). We implemented a business mentorship activity focusing on SEs in the project - Cereal System Initiative for South Asia. The business mentoring (BM) was implemented covering 10 SEs during 2014 to 2017 using discovery sensitization and facilitation mode. The key actions were, SWOT analysis, mentorship to develop strategic business plan, technical facilitation for seed quality improvement through maintenance breeding and on-farm farmer participatory trials as well as large plot demonstrations of recently released and pre-released wheat varieties. This resulted in 45% average increase in the volume of seed sold by individual SEs in three years. Five SEs started maintenance breeding in wheat and by third year, over 50% of the seed sold by the enterprises consisted of newly released varieties compared to 20% before mentoring. The improved performance of SEs prompted seed quality control authority of Nepal to issue a license to two of these private seed companies for maintenance breeding cum foundation seed production. As a consequence, foundation seed production of these entities increased by 60% in three years, and two of them have also started maintenance breeding in rice from 2017. The facilitation in developing business plan by SEs helped them to attract financial resources from different sources in upgrading seed processing, storage and laboratory facilities. Moreover, the National Wheat Research Program of Nepal has proposed the release of a new wheat variety BL 4341 through integration of on-farm trials and seed production data of these SEs. This new variety is resistant to major diseases, yields 7% higher than the popular cultivar NL 297 which was released 32 years ago and needed replacement due to susceptibility to wheat rusts


Large scale pre-breeding efforts for broadening gene pool and genetic improvement of wheat

BGRI 2018 Poster Abstract
Sukhwinder Singh CIMMYT
Prashant,Vikram, Deepmala, Sehgal, Juan, Burgueno, Carolina, Sansaloni, Cynthia, Ortiz, Ernesto, Solis, Lulu, Ledesma, Pillar, Suaste, G, Fuentes, J, Ireta, A, Sharma, P, Srivastava, Sridhar, Bhavani, Thomas, Payne, V, Govindan

Wheat breeding programs have successfully harnessed the potential of elite germplasm pool and have contributed significantly to global food security. However, to obtain additional genetic gain, useful diversity for key traits from landraces, synthetics and wild relatives should be incorporated in breeding germplasm pool. Maladaptation and linkage drags are the bottlenecks in utilizing these exotic genepools for pre-breeding. A systematic, focused, large scale effort has been pursued at CIMMYT through a three-way cross (exotic x elite1 x elite2) population development strategy. Population was advanced through selected-bulk scheme in way to select relevant genetic diversity while maintaining large population sizes. A total of 984 advanced pre-breeding lines (PBLs) were evaluated in multiple environments for grain yield related traits, micronutrient content and diseases resistance (yellow rust, stem rust, powdery mildew, and karnal bunt). Potential useful lines for these traits have been identified. High-density genomic characterization of PBLs, parental elites and exotics was conducted through a "haplotype map" based approach, which revealed 16% (58/361) exotic specific haplotype block (HB) introgression in PBLs. Out of 58 exotic specific HBs, 12 (12/361 = 3%) were found associated with traits evaluated in the study. Three HBs, H1.28 (1A), H18.1 (6D) and H5.23 (2B) were significantly important as they showed consistent effects across environments for grain yield (1A and 6D) and yellow rust (2B). This significant contribution of exotics into PBLs opens avenues to mine and utilize their useful alleles in wheat improvement. This research describes systematic large-scale pre-breeding efforts, as proof of concept of exotic germplasm deployment to the breeding pipelines simultaneously enriching genetic knowledge through high-density genomics analysis. Genetic knowledge coupled with breeding efforts should provide substantial gain required for next generation wheat varietal improvement.


Identification of resistant sources against rusts of wheat

BGRI 2018 Poster Abstract
Muhammad Fayyaz Crop Diseases Research Institute, National Agricultural Research Center, Park Road Islamabad, Pakistan
Anjum,Munir, Khalil, Ahmed Khanzad, Javed, Iqbal Mirza, Shahzad, Asad, Atiq ur, Rehman Rattu, Muahmmad, Imtiaz, , , , , , , , , , , , , , , , , ,

Evaluation of candidate lines to develop resistant varieties at multiplications in Pakistan is a regular activity which has been successfully done for many years. This approach assists in generation of future resistant cultivars around appropriate genes combinations thereby providing durable resistance outputs for wheat productivity. This year, National Uniform Wheat Yield Trial (NUWYT) comprised of 60 candidate lines. Among these 15 lines were also present in the last years NUWYT. The two years data revealed that there was only one line V-12066 resistant to all three rusts during the two consecutive seasons 2015-16 and 2016-17. Four candidate lines NR-487, V-122557, PR-115 and NRL-1123 were found resistant to yellow and leaf rust during 2015-16 and 2016-17. A candidate line DN-111 was found resistant to leaf and stem rust. There were three lines NW-1-8183-8, NW-5-20-1 and MSH which were found resistant to leaf rust only during two consecutive seasons. Similarly, two candidate lines V-122559 and QS-3 were found resistant to stem rust only, while one line NR-443 was resistant to yellow rust only. The present study provide the screening and evaluation system of Pakistan for promoting and releasing the resistant wheat varieties.


How to adapt durum wheat when the environment tries everything to kill it

BGRI 2018 Poster Abstract
Filippo Maria Bassi ICARDA, Rabat
Khaoula,El Hassouni, Priyanka, Gupta, Hafssa, Kabbaj, Meryam, Zaim, Amadou Tidiane, Sall, Bouchra, Belkadi, Ayed, Al-Abdallat, Ahmed, Amri, Rodomiro, Ortiz, Michael, Baum, , , , , , , , , ,

Durum wheat is the tenth most important crop in the world, but its cultivation is mostly limited to harsh, arid, and heat prone marginal lands. Breeding for tolerance to these conditions is often considered the most strategic approach to ensure adaptation, especially when paired with best agronomical practices. The word 'adaptation' summarizes all the research efforts conducted to identify the many traits controlling the mechanisms for withstanding or escaping the traceries of the environment. It can be summarized as "GGE vs E". The durum wheat breeding program of ICARDA deploys targeted phenotyping methods in combination with genomic scans to dissect these 'adaptive' traits into simple loci. These loci can then be pyramided via a combination of international field testing, markers assisted selection, genetically-driven crossing schemes, and genomic selection to derive climate-ready cultivars. Here, several examples of this approach are presented and their implications for 'adaptation' are discussed.