All BGRI Abstracts

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Race analysis of Puccinia striiformis f.sp. tritici in Iran-2017

BGRI 2018 Poster Abstract
Farzad Afshari Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
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Stripe rust of wheat, caused by Puccinia striiformis f. sp. trirtici (Pst) is an important disease in many parts of Iran. Over last two decades several epidemics have occurred in Iran causing the breakdown of widely utilized sources of resistance in wheat cultivars. Fifty isolates were collected from different parts of Iran during 2017. Eight isolates of Pst. from 2017 have been processed to date for race analysis. Infection types were assessed on a 0-9 scale 16 and 18 days after inoculation using a scale similar to that described by McNeal et al. (1971). Infection types (ITs) 7 to 9 were regarded as virulent (susceptible) and lower than 7 were avirulent. Pathotypes 102E158A+,Yr27; 6E158A+,Yr27; 102E158A+,Yr27; 166E154A+,Yr27; 38E174A+; 38E158A+,Yr27; 238E190A+,Yr27 and 38E190A+,Yr27 were identified. Pathotype 238E190A+,Yr27+ (from West of Iran) was more aggressive during this study. Plants with Yr1, Yr4, Yr5, Yr10, Yr15, Yr24 and YrSP were resistant to all pathotypes. Pathotypes with virulence on plant with gene/s Yr2, Yr6, Yr7, Yr8, Yr9, Yr25, Yr26, Yr27, Yr32, YrSD, YrSU, YrND and YrA were more common. Seedling tests of Iranian wheat cultivars to race 238E190A+,Yr27+ showed that the new released cultivars that included Parsi, Baharan, Bahar, Pishgam, Zareh, Urom, Maihan, Dena, Haydarei and Shabrang were resistant to the new aggressive race with virulence on plants with Yr27.

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Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection

BGRI 2018 Poster Abstract
Adnan Riaz The University of Queensland, Queensland Alliance for Agriculture and Food Innovation (QAAFI)
Naveenkumar,Athiyannan, Sambasivam, Periyannan, Olga, Afanasenko, Olga, Mitrofanova, Gregory, Platz, Elizabeth, Aitken, Rod, Snowdon, Evans, Lagudah, Lee, Hickey, Kai, Voss-Fels, , , , , , , , , ,

Leaf rust (LR) is an important wheat disease and deployment of resistant cultivars is the most viable strategy to minimise yield losses. We evaluated a diversity panel of 295 bread wheat accessions from the N. I. Vavilov Institute of Plant Genetic Resources (VIR), St Petersburg, Russia for LR response and performed genome-wide association studies (GWAS) using 10,748 polymorphic DArT-seq markers. The diversity panel was evaluated at the seedling and adult plant growth stages using three prevalent Australian P. triticina pathotypes. GWAS applied to 11 phenotypic data sets identified a total of 52 significant marker-trait associations representing 31 quantitative trait loci (QTL). Among them, 29 QTL were associated with adult plant resistance (APR). Of the 31 QTL, 13 were considered potentially new loci, whereas 4 co-located with previously catalogued Lr genes and 14 aligned to regions reported in other GWAS and genomic prediction studies. One seedling LR resistance QTL located on chromosome 3A showed pronounced levels of linkage disequilibrium among markers (r2 = 0.7), indicative of a high allelic fixation. Subsequent haplotype analysis for this region found 7 haplotype variants, of which 2 were strongly associated with LR resistance at the seedling stage. Similarly, analysis of an APR QTL on chromosome 7B revealed 22 variants, of which 4 were associated with resistance at the adult-plant stage. Most of the lines in the diversity panel carried 10 or more combined resistance-associated marker alleles, highlighting the potential of allele stacking for long-lasting resistance.

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Genome wide association mapping of resistance to leaf rust disease in wheat

BGRI 2018 Poster Abstract
Mohamed Mergoum The University of Georgia (UGA)
Suraj Sapkota, James Buck, Jerry Johnson, John Youmans

Leaf rust disease, caused by the fungal pathogen Puccinia triticina, is a major biotic constraint of wheat production worldwide. Genetic resistance is the most effective, economic, and environmentally safe method to control and reduce losses caused by this disease. More than 70 leaf rust resistance genes have been identified and mapped to specific chromosomes; however, continuous evolution of new leaf rust races requires constant search for new sources of resistance with novel QTL/genes. The objectives of this study were to identify sources of resistance, and to map genomic loci associated with leaf rust resistance using genome wide association study (GWAS) approach. Phenotypic evaluation of 297 spring wheat genotypes against a prevalent race of leaf rust in Georgia revealed that most of the genotypes were susceptible, and only 24 genotypes were found resistant. Furthermore, GWAS detected 10 markers on chromosomes 2A, 2B, 6A, 7A, and 7B significantly associated with leaf rust resistance. A marker on chromosome 7AS was identified revealing a novel genomic region associated with leaf rust resistance. The new identified sources of resistance and QTL could be used in wheat breeding programs to improve leaf rust resistance.

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An ABA-induced sugar transporter gene TaSTP1 reinforces wheat susceptibility to Puccinia striiformis

BGRI 2018 Poster Abstract
Baoyu Huai State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling
Lijing Pang, Pu Yuan, Shoujun Hu, Jie Liu, Zhensheng Kang

Pathogens, whatever their types, develop at the expense of the nutrients generated by host and it is largely assumed that classical sources turn into sinks when colonized by pathogens. Sugar appears to be the major carbon and energy source transferred from the host to pathogens. Uptake, exchanges and competition for sugar, at biotrophic interfaces, are controlled by membrane transporters and their regulation patterns are essential in determining the outcome of plant-fungal interactions. However, mechanisms of transport and transporters involved in carbon partitioning between organisms are still poorly understood.
In this study, a wheat sugar transporter protein (STP) gene, TaSTP1, was cloned from a wheat-Puccinia striiformis f. sp. tritici (Pst) interaction cDNA library. Transcripts of TaSTP1 were up-regulated in wheat leaves that were infected by Pst or had experienced exogenous ABA and certain abiotic treatments. Heterologous mutant complementation in Saccharomyces cerevisiae revealed that TaSTP1 transports a broad-spectrum monosaccharides including glucose, fructose, mannose and galactose. Transient expression in Nicotiana benthamiana and Arabidopsis protoplasts suggested that TaSTP1 is localized in plasma membrane. Yeast two hybrid and bimolecular fluorescence complementation (BiFC) validated oligomerization of TaSTP1. Knocking down TaSTP1 using the barley stripe mosaic virus-induced gene silencing system reduced the susceptibility of wheat to the Pst virulent pathotype CYR31. Hyphal abnormality was significantly observed in VIGS plants. These results suggest that TaSTP1 may directly or indirectly participate in sugar transport in the wheat-Pst interactions and exert influence on suagr supply of Pst.

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Effect of Stem Rust (Puccinia graminis f.sp.tritici) on Quality of Durum Wheat (Triticum tu gidum) in Ethiopia

BGRI 2018 Poster Abstract
Ashenafi Degete Ethiopian Institute of Agricultural Research, Debre Zeit Research Centre
Alemayehu,Chala, , , , , , , , , , , , , , , , , , , , , , , , , , , ,

Stem rust caused by Puccinia graminis f.sp. tritici is one of the major biotic constraints of wheat production. The disease may cause substantial quantitative and qualitative yield losses. However, much of the work in Ethiopia on this pathosystem focuses on quantitative yield loss and qualitative losses are often overlooked. Hence the current research was designed with the objectives to evaluate the effect of stem rust on physical and chemical quality of durum wheat and assess the relationships between disease intensity and quality parameters. For this purpose, a factorial field experiment was conducted at Debre Zeit Agricultural Research Centre during main and off seasons of 2016/17. The experiment involved six durum wheat varieties (Denbi, Hitosa, Tob.66, Mukiye, Ude and Mengudo) with different level of resistance to stem rust, and three Tilt spray schedules of Tilt?250 E.C at 7, 14 and 21 days. The experiment was laid out in randomized complete block design in factorial arrangements with three replications and untreated checks were included for comparison purpose. Results revealed significant variations in disease parameters and crop performance among spray schedules, wheat varieties and their interactions. Stem rust severity was the lowest on moderately susceptible and susceptible varieties treated with the Tilt at 7th day schedule. The highest stem rust severity (46.67%) was recorded on variety Hitosa without Tilt spray. Without Tilt treatment Denbi variety accounts protein content of 15.67% which is a false protein. At 7th day spray schedule this variety showed 12.90 % of grain protein content which is normal. There was a significant positive correlation between grain protein and stem rust severity (0.31**). There was significant negative relationships between terminal stem rust severity and thousand kernel weight, hectolitre weight, seed size and yield during off and main seasons were resulted, respectively.

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Genetic Improvement in Quality, Grain Yield and Yield Associated Traits of Durum wheat (Triticum turgidum var.durum L.) in Ethiopia

BGRI 2018 Poster Abstract
Mekuria Dejene Ethiopian Institute of Agricultural Research

Information about changes associated with advances in crop breeding is essential for understanding yield-limiting factors and developing new strategies for future breeding programmes. Thirty-six durum wheat varieties released since 1966 were evaluated in three replications of the Randomized Complete Design at Debre Zeit and Akaki, Ethiopia during the 2016 cropping season to estimate the amount of genetic gain made over time in grain yield potential, yield-associated traits and in protein content. Analysis of variance revealed significant differences among varieties for all 16 quantitative traits, protein content and protein harvest in Kg ha-1 at each of the locations. Grain yield varied between 1.66t ha-1 for Arendato released in 1966 to 3.90t ha-1 for Megenagna released in 2012 with mean of 2.952t ha-1 at Debre Zeit. At Akaki yield range was between 2.45 and 5.04t ha-1 with mean of 3.992t ha-1. 25 varieties surpassed Arendato (3.754t ha-1) at this location. In the combined ANOVA significant difference between the varieties was observed only for spike length, spikelets spike-1, grains spikelet, grains spike-1, plant height, days to flowering, thousand grain weight and hectoliter weight. Varieties specifically adapted to only one of the locations, widely adapted varieties and varieties not adapted to any of the locations were identified. Regression analysis revealed that grain yield has increased by 22kg ha-1 year-1 since 1966; an increase of 40.6% over yield in 1966. This was accompanied with a significant decline of 11.4% in spike length, 6.7% in spikelets spike-1, 17.9% in protein content and 31.2% in protein yield ha-1 and a significant increase of 41.1% in grains spikelet-1, 32.9% in number of grains spike-1, 22.3% in thousand grain weight, 17.8% in grain filling period, 23.9% in seed growth rate, 40.1% in grain yield production rate, 7.9% in harvest index.

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Evolution of durum wheat from Moroccan landraces to improved varieties

BGRI 2018 Poster Abstract
Mona Taghouti INRA
Fatima Gaboun, Nasserlhaq Nsarellah, Keltoum Rhrib, Atmane Rochdi

Durum wheat landraces have constituted the main source of Moroccan wheat production until the first half of the last century. This local germplasm is still cultivated in less favorable environments particularly in mountains and sub-Saharan regions. In recent decades of the late 20th and early 21th centuries, the genetic improvement had led to the release of new durum wheat cultivars highly uniform and more productive. The present paper investigates the evolution of genetic variability in terms of productivity and quality related traits using an historical series of Moroccan durum wheat genotypes grouped according to their period of release into "Landraces/ Old cultivars," "Intermediate cultivars," and "Modern cultivars". A significant improvement was achieved in durum wheat Morroccan productivity. Modern cultivars exceed their predecessors in terms of productivity related traits. The genetic gain was clearly associated with a reduction in plant cycle and plant height lowering the straw yield which resulted in an increase of grain yield estimated to 15.42Kg/ha/year. However, results revealed a reduction in terms of almost all quality related traits; -0.12% per year for protein content, -0.30 % per year for gluten strength, -0.31% per year for yellow pigment content, and -0.19% per year for vitreousness. The results underline the important variability in grain quality attributes among landraces genotypes. This local germplasm may be used as sources of quality-improving attributes in durum wheat breeding program to develop new varieties combining both high productivity and grain quality.

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Identification and characterization of winter wheat germplasm resistant to stem rust in Kenya and Turkey

BGRI 2018 Poster Abstract
Beyhan Akin International Maize and Wheat Improvement Center (CIMMYT), P.K. 39 Emek 06511 Ankara, Turkey
Nilufer,Akci, Sridhar, Bhavani, Mesut, Keser, Fatih, Ozdemir, Ruth, Wanyera, Alexey, Morgounov, , , , , , , , , , , , , , , , , ,

A diverse set of winter wheat germplasm was screened for resistance to stem rust in large-scale trials in Kenya and Turkey during 2009-16. The study aimed to select resistant material and characterize types of resistance and possible genes, as well as evaluate agronomic traits and resistance to other diseases to select superior variety candidates and parental lines. The study material was comprised of various Facultative and Winter Wheat Observation Nurseries (FAWWON), which are developed and distributed by the International Winter Wheat Improvement Program (www.iwwip.org) in Turkey. More than 1600 global accessions were screened, with most evaluated for two years. Based on stem rust data from Kenya, more than 400 genotypes were identified exhibiting adequate levels of resistance to the Ug99 race group. The highest number of resistant lines originated from IWWIP (~170), USA (~100), Russia (~40), Iran (~30), Romania (~20), and South Africa (~20). Material was also tested at two sites in Turkey: Haymana (artificial inoculation) and Kastamonu (natural infection). There was no significant correlation between stem rust severities in Kenya and in Turkey, due to differences in stem rust pathotypes. However, a set of germplasm (more than 100 entries) has been identified as resistant in both countries. This set represents promising material as variety candidates and parental lines; another study is currently identifying the genes controlling the stem rust resistance in this population. IWWIP distributed stem rust resistant germplasm to its global collaborators during 2010-2015, in response to the threat from the Ug99 race group. New resistant germplasm combining broad adaptation, high yields, and resistance to other diseases is available on request.

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Race analysis of Puccinia graminis f. sp. tritici led to identification of the new race TTKTK, affecting Sr31 and SrTmp, in Iran

BGRI 2018 Poster Abstract
Ramin Roohparvar Seed and Plant Improvement Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
Ali Omrani

The basidiomycetous fungus, Puccinia graminis f. sp. tritici (Pgt) causes stem rust disease as one of the most destructive wheat pathogens, worldwide. TTKSK and other Pgt races under Ug99 race group are considered as major threats to wheat production in east Africa and CWANA region by defeating the stem rust resistance gene Sr31, while its ineffectiveness was reported in Iran in 2007. Race TKTTF of Pgt caused a severe stem rust epidemic in southern Ethiopia in 2013, and was spread to Europe through 2016 Sicily outbreak. This research describes race identification of Iranian isolates collected during the widespread distribution of stem rust in 2014-16. Purified urediniospores of 123 Pgt isolates were separately inoculated on seedlings of 20 North American differential wheat cultivars carrying different Sr resistance gene/s. Infection types were recorded at 14 days post inoculation (dpi) using Stakman et al. 0-4 scale. Based on the letter code nomenclature, we identified the Pgt races TKTTF, TTTTF, TTKSK, TTKTK, PKTTF, TKSTF, PKSTF, PKTTC, PTRTF, PTTTF, PKSTC, TTRTF, TKSTC and PKRTF in Iran. TKTTF and TTTTF were determined as prevalent Iranian Pgt races. This is the first report of race TTKTK, a new variant of Ug99 race group with virulence on Sr31 and SrTmp resistance genes, in Iran. Since TTKTK primarily occurred in south west of Iran, the migration route for this new race seems to be similar to race TTKSK. The high race variation observed in this study could indicate a high genetic diversity among P. graminis f. sp. tritici populations in Iran, as a wheat center of origin.

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Pathogenic diversity in Puccinia striiformis f. sp. tritici isolates from Pakistan

BGRI 2018 Poster Abstract
Javed Iqbal Mirza Crop Diseases Research Institute, PARC Substation, Murree Pakistan
Sufyan,Muhammad, Abid Majeed, Satti, Munir, Anjum, Fayyaz, Muhammad, Atiq ur Rehman, Rattu, Imtiaz, Muhammad, , , , , , , , , , , , , , , , , ,

225 Puccinia striiformis f.sp. tritici isolates collected from wheat growing areas of Pakistan during 2013-2016 were analyzed using 18 near isogenic yellow rust differentials. Seventy eight races were identified among collection in which 20 were common (n > 2). Rest of the races were very rare and encountered only once (n=1). Races 574212, 574232, 474232, 474233, 574213 and 434232 were most frequent (n> 15). Pathogenic diversity analysis of the collection reveal high diversity (H =3.57) of the P. striiformis population of pakistan. On the basis of phenotypic response to yellow rust genes, the most frequent races could be grouped into 5 diverse groups. Distinct grouping was also observed in rarely encountered isolates. Most of the races were highly complex and 80% isolates had complexity ranging from 8 to 11. Virulence frequency for Yr6, Yr7, Yr8, Yr17, Yr27, Yr43 & YrExp2 remained above 80% while that of Yr1, Yr9 and Yr44 remained over 40%. Partial virulence was detected for Yr5, while virulence to Yr10, Yr15, YrSP was found in < 4% isolates. Paper discuss spatial and temporal distribution of P. striiformis races in Pakistan.

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