All BGRI Abstracts

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Breeding for climate smart bread wheat varieties

BGRI 2018 Poster Abstract
Amna Kanwal Wheat Research Institute, Ayub Agricultural Research Institute,Faisalabad,Pakistan
Mehvish,Makhdoom, Javed, Ahmad, Makhdoom, Hussain, Iqra, Ghafoor, , , , , , , , , , , , , , , , , , , , , ,

Wheat crop is facing immense losses each year owing to climate change, eventually being major threat to global food security. So, the objective of the present study was to screening of advance lines under drought and heat stress conditions. In following study, 30 advance lines of wheat along with four checks(Faislabad-08, Millat-11, Galaxy-13 and ujala16) with three treatments (heat, drought, normal) were tested for different morphological (days to heading, plant height, days to maturity, biomass,1000 grain weight and grain yield) and physiological (canopy temperature at vegetative & reproductive stage, NDVI vegetative & reproductive), parameters. Biplot analysis depicted that V2, V3, V8, V14, V19, V25, and V30 showed the highest OP vector for grain yield in drought environment. Whereas, under heat conditions, V3, V4, V5, V10, V11, and V12 displayed their maximum longest vector for grain yield. Correlation analysis depicted that grain yield had non-significant correlation with canopy temperature (vegetative stage), normalized difference vegetation index (vegetative stage) canopy temperature (reproductive stage), plant height, days to heading and days to maturity under heat stress environment, while it had significant association with biomass and thousand grain weight. Under drought environment, grain yield had positive and significant correlation with biomass while on the other hand it had negative but significant association with normalized difference vegetation index (reproductive stage) and canopy temperature (reproductive stage). Best performing lines could be efficiently exploited in research programs to evade the perilous impact of climate change.

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New virulence of some Puccinia triticina races to the effective wheat leaf rust resistant genes Lr 9 and Lr 19 under Egyptian field conditions

BGRI 2018 Poster Abstract
Walid El-Orabey Plant Pathology Research Institute
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Leaf rust resistance genes Lr9 and Lr19 were previously highly effective against the most predominant races of Puccinia triticina in Egypt. In 2015/2016 growing season, susceptible field reaction was recorded on these two genes where rust severity reached about 40S for Lr9 and 5S for Lr19 under Egyptian field conditions at four locations i.e. El-Behira, El-Minufiya, El-Qalubiya and El-Fayom governorates. Eight leaf rust field samples were collected from these governorates (four from each of Lr9 and Lr19). Forty single isolates were derived from the collected samples of Lr9 and Lr19 (each with 20 isolates). Eight pathotypes were identified from Lr9 and only two pathotypes were identified from Lr19. The most frequent pathotypes virulent to Lr9 were KTSPT (30%) followed by TTTMS (25%). Moreover, the other pathotypes ranged from 5 to 10%. Whereas, the most frequent pathotype virulent to Lr19 was CTTTT (85%) and the lowest PKTST was 15%. Pathotypes i.e. PRSTT, NTKTS and TTTMS identified from Lr9 were more aggressive on most of the tested leaf rust monogenic lines, as they were virulent to 36, 35 and 35 lines, respectively from a total of 39 lines. The two pathotypes; PKTST and CTTTT identified from Lr19 were virulent to 36 and 35 lines, respectively. Moreover, leaf rust pathotypes i.e. NPTNK and PRSTT from Lr9 and PKTST from Lr19 were the most aggressive on the tested wheat cultivars at seedling stage. The Lr2a was the most effective leaf rust resistance genes against the tested pathotypes at adult plant stage. Wheat cultivars Misr 1, Misr 2 and Nubariya 1 were the most resistant cultivars against the tested pathotypes at adult plant stage.

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Monitoring the yellow rust pathogen in Algeria

BGRI 2018 Poster Abstract
Abdelkader Benbelkacem National Agronomic Research Institute of Algeria

Among the many biotic constraints to wheat production in Algeria, rusts and in particular yellow rust (Puccinia striiformis), are among the most prevalent diseases that occur mostly all over the northern part of the country. Yellow rust has become now sporadic due to the exploitation of effective resistant genes in different forms and combinations (from CIMMYT and ICARDA). Earlier, durable resistance was probably due to many genes, such Yr18, Yr9, Yr27 and Yr1.
Yellow rust appeared as epidemic in 2004, over 600 000 ha of bread wheat ; severity exceeded 70%. Yields from affected fields of Hidhab a susceptible variety did not exceed 5.2 q/ha, while resistant cultivars yielded up to 48 q/ha. Monitoring of the pathogen virulence factors and their changes provides basic information for the development of an early warning system. This experiment was carried out in 5 Eastern Algeria locations. 30 lines of a standard set of yellow rust and 14 near-isogenic lines from ICARDA were sown in 2-m rows in 2014 and 2015. According to the results, virulence on Heines Kolben (Yr2), Kalyansona (Yr2), Lee (Yr7), Avocet R (YrA), Federation*4/Kavkaz (Yr9), Yr6/6*Avocet ?S?, Yr7/6*Avocet ?S?, Yr9/6*Avocet "S", Yr17/6*Avocet "S", TP1295 (Yr25) and YrSU was common during those two seasons. The frequency of virulence on plants with Yr2, Yr6, Yr7, Yr9 or YrA and Yr27 was up to 80%. No virulence was observed on plants with Yr1, Yr3, Yr4, Yr5, Yr8, Yr10, Yr15 and Yr18 genes. This material was extensively used in our breeding programs and several new cultivars are in the on farm trials where a participatory selection approach is used. All resistant and performing new varieties are being spread for replacement of most old susceptible ones.

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Improvement of durum wheat salinity tolerance by intergeneric hybridization of Triticum durum x Hordeum marinum

BGRI 2018 Poster Abstract
Olfa Ayed-Slama National Agronomic Institute of Tunisia
FATMA,BEN JEMAA, HAJER, SLIM-AMARA, , , , , , , , , , , , , , , , , , , , , , , , , ,

Wild species with valuable genetic heritage was used long time ago in interspecific crosses to improve cultivated plants adaptation to environmental constraints. The objective of this study is to transfer the salinity tolerance of Hordeum marinum, a wild barley species, to three durum wheat varieties (Karim, Razzek and Nasr) by intergeneric crosses. In order to skip the incompatibility between these species, in vitro immature embryo rescue was performed using B5 medium (Gamborg et al., 1968). The results showed that the genotype has an important effect on the success of the crosses and the rate of regenerated plants. We have found that 34.21% of the embryos derived from hybridization Razzek x Hordeum marinum has regenerated haploid plantlets, 5.88% for Karim x Hordeum marinum cross, and 2.78% for Nasr x Hordeum marinum.
The obtained chromosomal stock of the hybrid haploid plants was doubled by colchicine treatment concentrated at 0.05%. The rate of doubled haploid plants were reduced after colchicine treatment to 26.32% for Razzek x Hordeum marinum cross, 0% for Karim crossed with Hordeum marinum and remained unchanged for Nasr x Hordeum marinum.
The doubled haploids obtained are subjected to salt stress (6-12 g/l) in order to evaluate their tolerance to salinity.

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Migration of Puccinia triticina hit renowned across countries and continents

BGRI 2018 Poster Abstract
Younas Sohail Department of Botany, Faculty of Biology, Government College Murree, Pakistan
Barkat Ali, Muhammad Fayyaz, Atiq ur Reman Rattu, Abdul Samad Mumtaz, Muhammad Imtiaz

The new arrival of wheat rust pathotypes through migration during wheat cropping season requires regular monitoring to secure wheat production. In the present study, we collected leaf rust (Puccinia triticina Eriks.) infected wheat leaves from three major wheat growing provinces of Pakistan in the year 2014 to assess the haplotype diversity of P. triticina (Pt) isolates. The rDNA ITS sequence data of collected isolates was used in NCBI BLAST analysis. The blast hits showed best matches with Pt accessions EU014050 (Iran), JN120331 (Iran), JX533577 (Iran), AY956549 (Iran), DQ417412 (Czech Republic), DQ417418 (Israel), DQ417413 (Slovakia) and AF511083 (Louisiana). However, in cluster analysis, the Pakistani isolates showed strong bootstrap support with only Iranian and Indian (races 77-5 & 104-4) accessions that indicated eastward migratory mode of Pt pathotypes in Pakistan through westerly wind patterns. The predominant genotype DQ417412 (similar in alignment with AY956549 from Iran) overcome the resistance of top Pakistan mega varieties Seher06, Inqilab91, Kiran95, SKD1, TJ83 and NIFA-Batoor. Hence, the ITS based information remains a rapid molecular tool for pathogen surveillance across countries and continents.

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Mining sources of resistance to stripe rust in bread and durum wheat landraces from ICARDA genebank collection

BGRI 2018 Poster Abstract
Kumarse Nazari Turkey-ICARDA Regional Cereal Rust Research Center (RCRRC), ICARDA, Menemen, Izmir, Turkey
Muhammad Massub Tehseen, Ezgi Kurtulus, Maha Al Ahmed, Ahmed Amri, Mariana Yazbek, Ali Shehadeh

In 2016 the bread wheat (BW) and durum wheat (DW) landrace accessions were evaluated against PstS2 and in 2017 against a mixture of PstS2 and warrior race in field inoculations at Izmir precision stripe rust phenotyping platform. Inoculation was carried out three times during seedling, tillering and booting stages using mixture of fresh spore and talcum powder. Adult-plant responses of tested accessions were recorded according to 0-9 scale once the flag leaf of the susceptible cultivar became fully susceptible. During 2016, out of 3319 BW accessions, 1135 (36%), 871 (28%) and 1133 (36%) were found resistant (1-3 scale), moderately resistant (4-6), and susceptible (7-9) to PstS2, respectively. Amongst the resistant accessions in 2016, 1043 (33%) remained resistant while 786 (25%) showed moderate resistant and 1310 (42%) became susceptible. In 2017, 43% of moderately resistant accessions showed susceptibility to warrior race and 57% remained resistant to moderately resistant. Within the susceptible accessions to PstS2 race in 2016, 22% showed resistance to the warrior race and the remaining were susceptible. In case of DW in 2016, 76% (553) of the accessions were resistant to PstS2, 23% (163) were moderately resistant and only 1% (7) were found susceptible. In 2017, 329 (46%) of the resistant accessions were found resistant, whereas 289 (40%) and 105 (15%) showed moderately resistance and susceptible reaction to Warrior race, respectively. The present data indicated that BW landraces were generally more susceptible to stripe rust than DWs. Susceptibility of both BW and DW accessions to Warrior race indicated that most likely some of the uncharacterized resistance genes which conferred resistance to PstS2 were ineffective against the warrior race. Sources of resistance to both races were identified in both BW and DW. Genetic architecture of identified sources of resistance in present study requires further investigations.

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Evaluation of naked barley landraces from mountainous region of Nepal for yellow rust resistance.

BGRI 2018 Poster Abstract
Ajaya Karkee Nepal Agricultural Research Council, National Agriculture Genetic Resources Centre, Khumaltar, Lalitpur
Baidhya Nath,Mahto, Mina Nath, Paudel, Dhruba Bahadur, Thapa, Krishnahari, Ghimire, Bal Krishna, Joshi, Suraj, Baidya, Prem Bahadur, Magar, , , , , , , , , , , , , , , ,

Naked barley (Hordeum vulgare var. nudum L.), is an important winter crop grown in the mountain region of Nepal. Stripe rust (Puccinia striiformis f.sp. hordei), is the most destructive fungal disease of barley in the hills of Nepal with losses up to 100 %, occurring in cooler regions with higher altitude (1000-2500 m). Yield components along with final rust severity (FRS), area under disease progress curve (AUDPC) and average coefficient of infection (ACI) were evaluated for 20 indigenous barley accessions collected from mountainous region of Nepal at National Agriculture Genetic Resource Centre (Genebank), Khumaltar, Nepal during winter season of 2016-2017 with three replications. Barley cultivars displayed a range of severity from 0% to 100% with immune to susceptible reaction. AUDPC values were significantly different among the tested genotypes. Barley genotypes with accession number NGRC00837 (ACI-3), NGRC02357 (ACI-7), NGRC06026 (ACI-9) and NGRC02306 (ACI-12) were found resistant with lowest diseases progress while NGRC02350 (ACI-60), NGRC06036 (ACI-80), NGRC02312 (ACI-86), NGRC04003 (ACI-83) and NGRC02318 (ACI-93) were found as highly susceptible landraces. Correlation coefficients of agronomical parameters such as grains per spike and 1000-kernels weight with epidemiological parameters such as AUDPC and ACI were found highly significant. Resistant genotypes with low values for disease progress as well as diseases reaction were identified. The results indicate that source of resistance to yellow rust in naked barley genotypes are available in Nepal and can be used for resistant breeding in future.

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Mapping of all-stage leaf rust resistance genes in Triticum dicoccoides derived recombinant inbred line (RIL)

BGRI 2018 Poster Abstract
Ahmed Elkot School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004 India
Rohtas,Singh, Satinder, Kaur, Parveen, Chhuneja, , , , , , , , , , , , , , , , , , , , , , , ,

Leaf rust caused by Puccinia triticina is one of the most historical and economically important wheat diseases. Breeding for new cultivars with effective gene combinations is the most promising approach for reducing losses due to leaf rust. Wild emmer wheat, Triticum dicoccoides, the progenitor of modern tetraploid and hexaploid wheats, is an important resource for new variability for disease resistance genes. An accession of T. dicoccoides acc. pau4656 showed resistance against prevailing leaf rust races in India, when tested at the seedling and adult plant stage. The introgression line, developed from the cross of the leaf rust resistant T. dicoccoides acc. pau4656 and the susceptible T. durum cultivar Bijaga yellow, was crossed with T. durum cultivar PBW114 to generate recombinant inbred lines (RIL) for mapping leaf rust resistance gene(s). RIL population was screened against highly virulent leaf rust race 77-5 at seedling stage and inheritance analyses revealed the segregation of two leaf rust resistance genes. The genes have been temporarily designated as LrD1 and LrD2. A set of 387 SSR marker was used for bulked segregant analysis (BSA). The markers showing diagnostic polymorphism in the resistant and susceptible bulks were amplified on whole of the population. Single marker analysis using MapDisto software placed LrD1 on the long arm of chromosome 6A linked to the SSR marker Xwmc256 and LrD2 on long arm of chromosome 2A close to the SSR marker Xwmc632. T. durum cv. PBW114 used in the present study was also resistant to leaf rust at the seedling stage. So one of these leaf rust resistance genes might have been contributed by the PBW114 and other by T. dicoccoides. The current study identified valuable leaf rust resistance genes for deployment in wheat breeding programme.

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Occurrence of wheat rusts in Algeria and strategies to reduce crop losses

BGRI 2018 Poster Abstract
Amira Bentounsi University Mentouri of Constantine, Algeria

Wheat is the world's most widely grown food crop. New races of pathogens frequently overcome current resistant varieties. To address this issue Algeria has strategies for immediate action, medium term protection and long-term research efforts to develop new resistant wheat varieties. Yellow rust is a very important disease of wheat in Algeria where 60% of the wheat crop is grown under cooler high elevation climate conditions (2?C ? 15?C). Crop losses reached 80% during the 2004/2005 epidemics. Strategies adopted to reduce the risk of wheat rust are ongoing yearly surveillance, awareness, and early warning systems to farmers; and breeding and developing new varieties with high yield potential and durable resistance. Several highly resistant varieties (Tiddis, Boumerzoug, Massine, Akhamokh and Yacine) were selected and promoted following seed multiplication and commercial release. They are also widely used in crosses to improve local varieties. The newly released varieties are being distributed to farmers that grow susceptible varieties. This gene deployment will provide a natural barrier between eastern to western Algeria to intercept the major direction of air flow. Fungicide control is now routinely applied soon after rust detection or even preemptively. The level of awareness for wheat rusts across Algeria is now very high. Training among farmers for visual detection is widely promoted by plant protection and extension services. These strategies have been very effective in mitigating the threat of wheat stripe rust such that losses have not exceeded 10% over the last five years.

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Investigation on heat stress tolerance in bread wheat (Triticum aestivum. L) for the conditions of terminal heat stress.

BGRI 2018 Poster Abstract
Juned Bagwan Agharkar Research Institute Pune
yashavantha kumar,Kakanur, Shrikanth, Khairnar, Balgounda, Honrao, Vijendra, Baviskar, Ajit, Chavan, Vitthal, Gite, Deepak, Bankar, Sameer, Raskar, Satish chandra, Misra, , , , , , , , , , , ,

Heat stress globally remains the most important factor determining yield anomalies. Terminal heat stress shortens the duration of grain filling. Hence, this investigation was undertaken during the cropping season 2016-17 to evaluate heat stress tolerance of 32 bread wheat genotypes planted in timely (optimal temperature) and late (terminal heat stress) sown condition at Agharkar Research Institute, Pune. Data were collected and analyzed for various agronomical and physiological traits and also selection indices for stress tolerance, derived from grain yield of wheat genotypes under optimal and late sowing conditions. It was observed that the genotypes DBW 187, GW 477, HD 2932, DBW 107, PBW 752 were the highest yielding under timely sown condition whereas, HD 3226, DBW 187, HP 1963, HD 3219, DBW 196 were the highest yielding under late sown condition. DBW 187 was found to withstand the stress conditions. Minimum percent yield decrease and high yield stability index (YSI) was found in HD 3219 followed by HD 3226 and DBW 196 which indicated their better performance under stress condition. Harmonic mean, a stress tolerance selection index was found to be the best fit of linear model (R2 = 0.78) and a good indicator of high yield under heat stress condition. Physiological parameters, Chlorophyll (SPAD), canopy temperature (Infra-red thermometer) and vegetation index (NDVI) have not shown significant relation with yield, however, they were found to be significantly associated with yield contributing traits like biomass, thousand grain weight, grain number per spike. DBW 187 and HP 1963 showed stable yields with high PCA 1 and low PCA 2, indicating their resilience to stress conditions. The investigation has resulted in identification of genotypes for terminal heat stress conditions and also given greater insights in understanding the importance of physiological traits and stress tolerance indices in selection process.

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